NF-κB1 p105/p50 Antibody - CD BioSciences

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NF-κB1 p105/p50 Antibody

NF-κB1 p105/p50 Antibody

SPA-07926

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name NF-κB
Gene Abbr. NFKB1
Gene ID 4790
Full Name nuclear factor kappa B subunit 1
Alias CVID12, EBP-1, KBF1, NF-kB, NF-kB1
Introduction Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus.Following IKK-mediated phosphorylation of p105 NF-κB at multiple sites (Ser921, 923, 927, and 932) on its carboxy-terminus, SCF/β-TrCP mediated processing produces the 50 kDa active form p50.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. A2-7
Isotype IgM
Immunogen Synthetic peptide (KLH-coupled).
Usage
Application WB, IF, IHC
Dilutions Western Blot (1:1000-1:2000); Immunofluorescence (1:200); Immunohistochemistry (1:200-1:500)
MW(KDa) 50 (mature), 120 (precursor)
Reactivity Human, Mouse
Storage & Handling
Storage Buffer TBS (pH 7.4), 1% BSA, 40% Glycerol.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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