NEDD4 Knockout Cell Line - CD BioSciences

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NEDD4 Knockout Cell Line

NEDD4 Knockout Cell Line

SPL-02265

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Description
7bp deletion
Target Information
Target Name NEDD4
Gene Abbr. NEDD4
Gene ID 4734
Full Name NEDD4 E3 ubiquitin protein ligase
Alias NEDD4-1, RPF1
Species Human
Genomic Locus chr15:55862956
Transcript NM_006154
WT Expression Level 8.38 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 7bp deletion in a coding exon of NEDD4.
Description 7bp deletion
Parental Cell Line C631
Guide RNA Sequence TTACATAATAGGTCCTTCCA
PCR Primer Forward: AGTCCTGGGCAATAAAAATCCTAGT
Reverse: GTAAACTTAATTGCTGCCATCCTGT
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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