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Naked1 Antibody
SPA-07828
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Naked1 |
| Gene Abbr. | NKD1 |
| Gene ID | 85407 |
| Full Name | NKD inhibitor of WNT signaling pathway 1 |
| Alias | Naked1 |
| Introduction | Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism. Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium. Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway. Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway. Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Gln227 of human Naked1. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln227 of human Naked1. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 59, 61 |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | Naked1 antibody detects endogenous levels of total Naked1 proteins. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.