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Miro2 Antibody
SPA-07581
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Miro2 |
| Gene Abbr. | RHOT2 |
| Gene ID | 89941 |
| Full Name | ras homolog family member T2 |
| Alias | ARHT2, C16orf39, MIRO-2, MIRO2, RASL |
| Introduction | Mitochondrial Rho GTPase 1 (Miro1, RHOT1) and mitochondrial Rho GTPase 2 (Miro2, RHOT2) are atypical Ras GTPase proteins that localize to the outer mitochondrial membrane. These evolutionarily conserved proteins contain GTP-binding domains and a pair of calcium-binding EF hand domains. Research studies indicate that Miro1 and Miro2 function in the axonal transport of mitochondria in neurons. Both Miro proteins play an essential role in mitochondrial trafficking by attaching mitochondria to essential motor and adaptor proteins. Miro GTPase proteins that are anchored to the outer mitochondrial membrane interact with kinesin-binding proteins TRAK1 and TRAK2 to provide a link between mitochondria to microtubules. Increased levels of synaptic calcium appears to inhibit mitochondrial trafficking mediated by Miro, suggesting a role for the EF hand as a calcium sensor. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Gly452 of human Miro2 protein. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly452 of human Miro2 protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 80 |
| Reactivity | Human, Monkey |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total Miro2 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.