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MIF Knockout Cell Line
SPL-02114
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 74bp insertion |
| Target Information | |
|---|---|
| Target Name | MIF |
| Gene Abbr. | MIF |
| Gene ID | 4282 |
| Full Name | macrophage migration inhibitory factor |
| Alias | GIF, GLIF, MMIF |
| Species | Human |
| Genomic Locus | chr22:23894515 |
| Transcript | NM_002415 |
| WT Expression Level | 181.94 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 74bp insertion in a coding exon of MIF. |
| Description | 74bp insertion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | GAGGAACCCGTCCGGCACGG |
| PCR Primer |
Forward: CACAGTGGTGTCCGAGAAGTC Reverse: ATGTACTGCGAGGAAAGGGC |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.