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MAP3K14 Knockout Cell Line
SPL-01987
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 29bp deletion |
| Target Information | |
|---|---|
| Target Name | NIK |
| Gene Abbr. | MAP3K14 |
| Gene ID | 9020 |
| Full Name | mitogen-activated protein kinase kinase kinase 14 |
| Alias | FTDCR1B, HS, HSNIK, NIK |
| Species | Human |
| Genomic Locus | chr17:45290499 |
| Transcript | NM_003954 |
| WT Expression Level | 1.30 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq, Jul 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 29bp deletion in a coding exon of MAP3K14. |
| Description | 29bp deletion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | TGCCATCTCTATCATCGCCC |
| PCR Primer |
Forward: TGTAAAACGACGGCCAGTCAGGATGAGCACAAGCCTG Reverse: TACCTCTCTTCCCCTGCTCC |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.