Online Inquiry
MAP2K4 (-/-) Cell Line
SPL-01971
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| MAP2K4 (-/-) |
| Target Information | |
|---|---|
| Target Name | SEK1/MKK4 |
| Gene Abbr. | MAP2K4 |
| Gene ID | 6416 |
| Full Name | mitogen-activated protein kinase kinase 4 |
| Alias | JNKK, JNKK1, MAPKK4, MEK4, MKK4 |
| Species | Human |
| Genomic Locus | 17p12 |
| Introduction | This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. |
| Product Details | |
|---|---|
| Cell Line Model | Panc 04.03 |
| Genotype | MAP2K4 (-/-) |
| Description | MAP2K4 (-/-) |
| Parental ATCC ID | CRL-2555 |
| STR Profile | Amelogenin: X; CSF1PO:12; D13S317: 11, 12; D16S539: 11,12; D5S818: 11; D7S820: 8, 12; THO1: 9.3; TPOX: 10, 11; vWA: 14,16; ATCC |
| Disease | Adenocarcinoma |
| Tissue | Pancreatic |
| Gender | Male |
| Key Endogenous Mutations | KRAS |
| CVT Selection Condition | No antibiotic resistance |
| Cell Number | 1x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:2-1:6. |
| Culture Medium | RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:2-1:6. |
| Freeze Medium | Complete growth media 45% + USDA approved FBS 50% + DMSO 5% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.