Online Inquiry
MAD2L1 Knockout Cell Line
SPL-01932
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| 10bp deletion |
| Target Information | |
|---|---|
| Target Name | Mad-2 |
| Gene Abbr. | MAD2L1 |
| Gene ID | 4085 |
| Full Name | mitotic arrest deficient 2 like 1 |
| Alias | HSMAD2, MAD2 |
| Species | Human |
| Genomic Locus | chr4:120066677 |
| Transcript | NM_002358 |
| WT Expression Level | 129.25 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing) |
| Introduction | MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | HAP1 |
| Genotype | HAP1 cell line, edited by CRISPR/Cas to contain a 10bp deletion in a coding exon of MAD2L1. |
| Description | 10bp deletion |
| Parental Cell Line | C631 |
| Guide RNA Sequence | CGATTTCGGCGCTCCCGCGC |
| PCR Primer |
Forward: GGATCTGCACTTAAAGGAAGAAACG Reverse: CGTCGTTACTTTTGAAACGCTTG |
| Handling Specifications | |
|---|---|
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish. |
| Culture Medium | IMDM + 10% FCS |
| Growth Properties | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Freeze Medium | IMDM + 20% FCS + 10% DMSO |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.