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LIG4 (-/-); TP53 (-/-) Cell Line
SPL-01881
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| LIG4 (-/-); TP53 (-/-) |
| Target Information | |
|---|---|
| Target Name | LIG4 |
| Gene Abbr. | LIG4 |
| Gene ID | 3981 |
| Full Name | DNA ligase 4 |
| Alias | LIG4S |
| Species | Human |
| Genomic Locus | 13q33-q34;17p13.1 |
| Introduction | The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | NALM-6 |
| Genotype | LIG4 (-/-); TP53 (-/-) |
| Description | LIG4 (-/-); TP53 (-/-) |
| Parental ATCC ID | DSMZ: ACC 128 |
| STR Profile | Amelogenin: X,Y; CSF1PO: 11,12; D13S317: 8,11; D16S539: 12,13; D5S818: 13; D7S820: 10,12; THO1: 7,9.3; TPOX: 8,11; vWA: 18,19; ATCC |
| Disease | Acute lymphoblastic leukemia |
| Tissue | B-Cell |
| Gender | Male |
| Key Endogenous Mutations | CDKN2A, CDKN2a(p14), MSH2, NRAS |
| CVT Selection Condition | Hygromycin resistant - 0.4 mg/mL; G418 resistant - 1.5 mg/mL |
| Cell Number | 5x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a suspension at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Maintain at 1.0-3.0 x 106 cells/ml, splitting approximately 1:6-1:10 |
| Culture Medium | RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a suspension at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Maintain at 1.0-3.0 x 106 cells/ml, splitting approximately 1:6-1:10 |
| Freeze Medium | Complete growth media 40% + USDA approved FBS 50% + DMSO 10% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.