Leukemia inhibitory factor Sandwich ELISA Kit - CD BioSciences

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Leukemia inhibitory factor Sandwich ELISA Kit

Leukemia inhibitory factor Sandwich ELISA Kit

SPK-00807

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Target Information
Target Name LIF
Gene Abbr. Lif
Gene ID 60584
Full Name LIF interleukin 6 family cytokine
Introduction LIF (Leukemia inhibitory factor; also Differentiation-stimulating factor) is a 22 kDa (predicted) glycoprotein, member of the leukemia inhibitory factor/interleukin-6 (LIF/IL-6) family of cytokines. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, it is produced by a variety of cells including T cells, monocytes, fibroblasts, osteoblasts and mast cells. LIF induces hematopoietic differentiation in normal and myeloid leukemia cells, neuronal cell differentiation and stimulation of acute-phase protein synthesis in hepatocytes. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family. Human and mouse LIF exhibit 78% aa sequence identity.
Product Details
Measurement Sandwich
Usage
Application ELISA
Reactivity
Assay Principle quantitative
Sensitivity 0.078 ng/mL
Detection Range 0.312 ng/mL - 20 ng/mL
Sample Volume 50-100μL
Assay Time 1-5h
Detection Wavelength 450 nm
Precision Intra-assay Precision (Precision within an assay): CV%<8%.
Inter-assay Precision (Precision between assays): CV%<10%.
Storage & Handling
Storage Temp. Store at 2-8°C. Please refer to protocol.

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