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Leukemia inhibitory factor Sandwich ELISA Kit
SPK-00807
Size | Price |
1 X 96T | Online Inquiry |
5 X 96T | Online Inquiry |
10 X 96T | Online Inquiry |
More Options | Online Inquiry |
Target Information | |
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Target Name | LIF |
Gene Abbr. | Lif |
Gene ID | 60584 |
Full Name | LIF interleukin 6 family cytokine |
Introduction | LIF (Leukemia inhibitory factor; also Differentiation-stimulating factor) is a 22 kDa (predicted) glycoprotein, member of the leukemia inhibitory factor/interleukin-6 (LIF/IL-6) family of cytokines. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, it is produced by a variety of cells including T cells, monocytes, fibroblasts, osteoblasts and mast cells. LIF induces hematopoietic differentiation in normal and myeloid leukemia cells, neuronal cell differentiation and stimulation of acute-phase protein synthesis in hepatocytes. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family. Human and mouse LIF exhibit 78% aa sequence identity. |
Product Details | |
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Measurement | Sandwich |
Usage | |
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Application | ELISA |
Reactivity | |
Assay Principle | quantitative |
Sensitivity | 0.078 ng/mL |
Detection Range | 0.312 ng/mL - 20 ng/mL |
Sample Volume | 50-100μL |
Assay Time | 1-5h |
Detection Wavelength | 450 nm |
Precision | Intra-assay Precision (Precision within an assay): CV%<8%. Inter-assay Precision (Precision between assays): CV%<10%. |
Storage & Handling | |
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Storage Temp. | Store at 2-8°C. Please refer to protocol. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.