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JMJD3 Antibody
SPA-06770
| Size | Price |
| 100 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | JMJD3 |
| Gene Abbr. | KDM6B |
| Gene ID | 23135 |
| Full Name | lysine demethylase 6B |
| Alias | JMJD3, NEDCFSA |
| Introduction | The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development. Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins. The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate. Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families. The three members of the UTX/UTY family include the ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX), the ubiquitously transcribed Y chromosome tetratricopeptide repeat protein (UTY) and JmjC domain-containing protein 3 (JMJD3). This family of proteins has been shown to demethylate both di- and tri-methyl histone H3 Lys 27. The UTX gene escapes X inactivation in females and is ubiquitously expressed. UTX functions to regulate HOX gene expression during development. JMJD3 functions to regulate gene expression in macrophages responding to various inflammatory stimuli and has been shown to be upregulated in prostate cancer. Both UTX and JMJD3 interact with mixed-lineage leukemia (MLL) complexes 2 and 3, both of which have been shown to methylate histone H3 at Lys4. The UTY gene is expressed in most tissues in the male mouse. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | A peptide corresponding to 16 amino acids near the carboxy terminus of human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. The immunogen is located within amino acids 1370-1420 of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3. Amino Acid Sequence: KVPGSRTPGHQENNNF. |
| Usage | |
|---|---|
| Application | WB, ELISA, IF |
| Dilutions | Western Blot (0.25-4 µg/mL); Immunofluorescence (2.5-5 µg/mL); Immunofluorescence (2.5 µg/mL); Immunofluorescence (20 µg/mL) |
| MW(KDa) | 200 |
| Reactivity | Human, Mouse, Rat |
| Specificity | This antibody is specific for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 and will not recognize other JMJD proteins. Human Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has 2 isoforms, including isoform 1 (1682aa, 181kD) and isoform 2 (1643aa, 177kD). Mouse Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 has one isoform (1641aa, 176kD) and rat Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 also has one isoform (1356aa, 146kD). It can detect human, mouse and rat. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.