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JAK2 (V617F/+) Cell Line
SPL-01750
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| JAK2 (V617F/+) |
| Target Information | |
|---|---|
| Target Name | Jak2 |
| Gene Abbr. | JAK2 |
| Gene ID | 3717 |
| Full Name | Janus kinase 2 |
| Alias | JTK10 |
| Species | Human |
| Genomic Locus | 9p24 |
| Introduction | This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]. |
| Product Details | |
|---|---|
| Cell Line Model | SW48 |
| Genotype | JAK2 (V617F/+) |
| Description | JAK2 (V617F/+) |
| Parental ATCC ID | CCL-231 |
| STR Profile | Amelogenin: X; CSF1PO: 9,10; D13S317: 11, 12; D16S539: 11,13; D5S818: 10, 14; D7S820: 9, 10; THO1: 6,9.3; TPOX: 8; vWA: 18,20, 21; ATCC |
| Disease | Colorectal adenocarcinoma, Dukes Type C Grade IV |
| Tissue | Colon |
| Gender | Female |
| Key Endogenous Mutations | CTNNB1, FBXW7, EGFR |
| CVT Selection Condition | G418 resistant - 0.8 mg/mL |
| Cell Number | 2x10^6 |
| Handling Specifications | |
|---|---|
| Revival | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:3-1:6. Cells may experience a lag period of approximately 1 week before stable growth is achieved. |
| Culture Medium | RPMI 1640 including 2 mM L-glutamine and 25 mM sodium bicarbonate, supplemented with 10% FBS. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:3-1:6. Cells may experience a lag period of approximately 1 week before stable growth is achieved. |
| Freeze Medium | Complete growth media 45% + USDA approved FBS 50% + DMSO 5% |
| Biosafety Level | BSL-1 |
| Disclaimer | This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.