Jag1 cDNA ORF Clone, Rat, In cloning vector - CD BioSciences

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Jag1 cDNA ORF Clone, Rat, In cloning vector

Jag1 cDNA ORF Clone, Rat, In cloning vector

SPD-09385

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1 Unit Online Inquiry
Description
Full length Clone DNA of Rat jagged 1.
Target Information
Species Rat
Target Name Jagged1
Gene Abbr. Jag1
Gene ID 29146
Full Name jagged canonical Notch ligand 1
Introduction Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch. Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4. Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions.Mutation in Jagged1 is associated with Alagille syndrome, an autosomal dominant disorder characterized by abnormal development of liver, heart, skeleton, eye, and face and Tetralogy of Fallot (ToF), a common form of complex congenital heart disease. Jagged1 expression is associated with prostate cancer metastasis and recurrence.
Product Details
Description Full length Clone DNA of Rat jagged 1.
NCBI Ref Seq NM_019147.1
RefSeq ORF Size 3660 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence except for the point mutations: 3557 T/C,3562-3564 CAG/AGC, 3565 C/G, resulting in the amino acid Val substitution by Ala, Gln substitution by Ser, Arg substitution by Gly and 636 C/T,1761 T/G,1944 T/C not causing the amino acid variation.
Vector pGEM-T Vector
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers SP6 and T7 or M13-47 and RV-M
Antibiotic in E.coli Ampicillin
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

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