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ITCH Antibody
SPA-06618
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | ITCH |
| Gene Abbr. | ITCH |
| Gene ID | 83737 |
| Full Name | itchy E3 ubiquitin protein ligase |
| Alias | ADMFD, AIF4, AIP4, NAPP1 |
| Introduction | ITCH is a HECT domain-containing E3 ubiquitin ligase, first identified in genetic studies of the mouse agouti locus, in which mutations result in characteristic coat color changes. One particular agouti mutation (non-agouti-lethal 18H) is notable for the development of immunological defects not observed in other agouti mutant mice; these include lymphoid hyperplasia and chronic stomach, lung and skin inflammation (manifest as constant itching). The 18H agouti mutation was traced to a chromosomal inversion that disrupted expression of an adjacent gene in the agouti locus, subsequently termed Itch to reflect the chronic itching phenotype.Further characterizations revealed that Itch encoded a NEDD4-like E3-ubiquitin ligase capable of catalyzing Lys29, Lys48, and/or Lys63-linked ubiquitination of target proteins, leading to their degradation by the proteosome pathway. The distinct phenotypes of Itch mutant mice led to the identification of an important regulatory role for ITCH-mediated ubiquitination in inflammatory signaling pathways. For example, ITCH-mediated ubiquitination of the transcription factor JunB was shown to play a direct inhibitory role in regulating expression of the proinflammatory cytokine IL-4. ITCH-null T lymphocytes consequently exhibit increased production of IL-4, leading to biased differentiation of naive CD4+ cells towards the proinflammatory Th2 lineage. In accordance with the findings from mutant Itch mouse models, a genetic linkage study in humans identified loss-of-function mutations in ITCH as a direct cause of syndromic multisystem autoimmune disease (SMAD).Notably, targets of ITCH-mediated ubiquitination are not restricted to immune signaling pathways. For example, key mediators of the Hedgehog, Wnt/β-catenin Hippo and Notch signaling pathways have been identified as important targets of ITCH-mediated ubiquitination. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D8Q6D |
| Immunogen | Synthetic peptide corresponding to residues surrounding Asp125 of human ITCH protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp125 of human ITCH protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:200) |
| MW(KDa) | 105 |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total ITCH protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.