Insr cDNA ORF Clone, Rat, N-FLAG tag - CD BioSciences

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Insr cDNA ORF Clone, Rat, N-FLAG tag

Insr cDNA ORF Clone, Rat, N-FLAG tag

SPD-08709

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1 Unit Online Inquiry
Description
Full length Clone DNA of Rat insulin receptor with N terminal Flag tag.
Target Information
Species Rat
Target Name InsR
Gene Abbr. Insr
Gene ID 24954
Full Name insulin receptor
Introduction The heterotetrameric receptors for insulin (INS R) and IGF-I (IGF-I R) are receptor tyrosine kinases that consist of two ligand-binding alpha subunits and two beta subunits. Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits. Phosphorylation of Tyr1162 and 1163 on INS R and Tyr1135 and 1136 on IGF‑I R stimulates intrinsic kinase activity.
Product Details
Description Full length Clone DNA of Rat insulin receptor with N terminal Flag tag.
NCBI Ref Seq NM_017071.2
RefSeq ORF Size 4155 bp
Vector pCMV3-SP-N-FLAG
Promoter Enhanced CMV promoter
Tag Sequence FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.