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iNOS Antibody
SPA-06022
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | iNOS |
| Gene Abbr. | NOS2 |
| Gene ID | 4843 |
| Full Name | nitric oxide synthase 2 |
| Alias | HEP-NOS, INOS, NOS, NOS2A |
| Introduction | Nitric Oxide Synthase (NOS) catalyzes the formation of nitric oxide (NO) and citrulline from L-arginine, oxygen, and cofactors. Three family members have been characterized: neuronal NOS (nNOS), which is found primarily in neuronal tissue; inducible NOS (iNOS), which is induced by interferon gamma and lipopolysaccharides in the kidney and cardiovascular system; and endothelial NOS (eNOS), which is expressed in blood vessels. NO is a messenger molecule with diverse functions throughout the body including the maintenance of vascular integrity, homeostasis, synaptic plasticity, long-term potentiation, learning, and memory.Nitric oxide catalyzed by iNOS is involved in host defense against protozoa, bacteria, fungi, and viruses. Unlike constitutively expressed eNOS and nNos, iNOS is not usually expressed in quiescent cells. iNOS is transcriptionally induced in response to bacterial endotoxins, such as LPS and proinflammatory cytokines, in macrophages and various other cell types. Transcription factors involved in iNOS transcription include NF-κB, AP-1, and STAT. Different signaling pathways either promote (Jak1/2, PKC, c-Raf, p38 MAP kinase, and p44/42 MAP kinase) or inhibit (PI3 kinase) iNOS expression depending on stimulus and cell type. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | INOS Antibody was made to a synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS. |
| Usage | |
|---|---|
| Application | WB, FC, IF, IHC |
| Dilutions | Western Blot (1:200-1:800); Immunofluorescence (1:50); Immunohistochemistry (1:20) |
| MW(KDa) | 130 |
| Reactivity | Human, Mouse, Rat, Porcine |
| Specificity | This antibody detects iNOS. It does not detect other NOS isoforms. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.