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ILK1 Antibody
SPA-05996
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | ILK1 |
| Gene Abbr. | ILK |
| Gene ID | 3611 |
| Full Name | integrin linked kinase |
| Alias | HEL-S-28, ILK-1, ILK-2, P59, p59ILK |
| Introduction | Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin. ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin. These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP. It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Peptide with sequence C-DMIVPILEKMQDK corresponding to C-Terminus according to NP_004508.1, NP_001014794.1, NP_001014795.1. |
| Usage | |
|---|---|
| Application | WB, IHC, ELISA |
| Dilutions | Western Blot (0.01-0.03 µg/mL); Immunohistochemistry (2-4 µg/mL); ELISA (1:64000) |
| MW(KDa) | 51 |
| Reactivity | Human, Mouse, Rat, Porcine, Bovine, Canine |
| Specificity | All 3 varients (represented by NP_004508.1; NP_001014794.1 and NP_001014795.1) encode the same protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.