Il4 cDNA ORF Clone, Mouse, C-HA tag - CD BioSciences

service-banner

Il4 cDNA ORF Clone, Mouse, C-HA tag

Il4 cDNA ORF Clone, Mouse, C-HA tag

SPD-08351

Size Price
1 Unit Online Inquiry
Description
Full length Clone DNA of Mouse interleukin 4 with C terminal HA tag.
Target Information
Species Mouse
Target Name IL-4
Gene Abbr. Il4
Gene ID 16189
Full Name interleukin 4
Alias BSF-1, Il, Il-4
Introduction Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities.
Product Details
Description Full length Clone DNA of Mouse interleukin 4 with C terminal HA tag.
NCBI Ref Seq NM_021283.1
RefSeq ORF Size 465 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence.
Vector pCMV3-C-HA
Promoter Enhanced CMV promoter
Tag Sequence HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Restriction Sites KpnI + XbaI (6kb + 0.47kb)
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.