Il25 cDNA ORF Clone, Mouse, C-HA tag - CD BioSciences

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Il25 cDNA ORF Clone, Mouse, C-HA tag

Il25 cDNA ORF Clone, Mouse, C-HA tag

SPD-08139

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Description
Full length Clone DNA of Mouse interleukin 25 with C terminal HA tag.
Target Information
Species Mouse
Target Name IL-25
Gene Abbr. Il25
Gene ID 140806
Full Name interleukin 25
Alias IL-1, IL-17e, Il1, Il17e
Introduction A number of cytokines belonging to the interleukin (IL)-17 family have been identified, termed IL-17A-F. IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis, multiple sclerosis, and promotion of tumor growth. IL-17E, C, and B are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that an additional IL-17R related receptor may exist. Receptors for IL-17B and IL-17E have been independently isolated by Shi, et al and Lee, et al. and have been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E, also called IL-25, induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.
Product Details
Description Full length Clone DNA of Mouse interleukin 25 with C terminal HA tag.
NCBI Ref Seq NM_080729.2
RefSeq ORF Size 510 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence.
Vector pCMV3-C-HA
Promoter Enhanced CMV promoter
Tag Sequence HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Restriction Sites HindIII + XbaI (6kb + 0.55kb)
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

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