IL15 cDNA ORF Clone, Human, C-FLAG tag - CD BioSciences

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IL15 cDNA ORF Clone, Human, C-FLAG tag

IL15 cDNA ORF Clone, Human, C-FLAG tag

SPD-07218

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Description
Full length Clone DNA of Human interleukin 15, transcript variant 1 with C terminal Flag tag.
Target Information
Species Human
Target Name IL-15
Gene Abbr. IL15
Gene ID 3600
Full Name interleukin 15
Alias IL-15
Introduction Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2. The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta/gamma c on adjacent cells. This enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing.
Product Details
Description Full length Clone DNA of Human interleukin 15, transcript variant 1 with C terminal Flag tag.
NCBI Ref Seq NM_172174.2
RefSeq ORF Size 489 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence.
Vector pCMV3-C-FLAG
Promoter Enhanced CMV promoter
Tag Sequence FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Restriction Sites KpnI + XbaI (6kb + 0.54kb)
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.