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Il12b cDNA ORF Clone, Rat, In cloning vector
SPD-07050
| Size | Price |
| 1 Unit | Online Inquiry |
| Description |
|---|
| Full length Clone DNA of Rat interleukin 12b. |
| Target Information | |
|---|---|
| Species | Rat |
| Target Name | IL-12 |
| Gene Abbr. | Il12b |
| Gene ID | 64546 |
| Full Name | interleukin 12B |
| Alias | Il12 |
| Introduction | Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. |
| Product Details | |
|---|---|
| Description | Full length Clone DNA of Rat interleukin 12b. |
| NCBI Ref Seq | NM_022611.1 |
| RefSeq ORF Size | 1008 bp |
| Sequence Information | Identical with the Gene Bank Ref. ID sequence except for the point mutation 505-506 CG/GC resulting in the amino acid Arg substitution by Ala. |
| Vector | pGEM-T Vector |
| Quality Control | The plasmid is confirmed by full-length sequencing. |
| Storage | The lyophilized plasmid can be stored at ambient temperature for three months. |
| Usage | |
|---|---|
| Sequencing Primers | SP6 and T7 or M13-47 and RV-M |
| Antibiotic in E.coli | Ampicillin |
| Shipping | Each tube contains lyophilized plasmid. |
| Storage | The lyophilized plasmid can be stored at ambient temperature for three months. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.