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IL-6 Antibody
SPA-05953
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IL-6 |
| Gene Abbr. | IL6 |
| Gene ID | 3569 |
| Full Name | interleukin 6 |
| Alias | BSF-2, BSF2, CDF, HGF, HSF |
| Introduction | Acute phase response is induced by interleukin-6 (IL-6) produced by T cells, macrophages, fibroblasts, endothelial and other cells. IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-β, is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization. gp130 homodimerization triggers the Jak/Stat cascade and the SHP-2/Erk MAP kinase cascade. IL-6 also forms a complex with an IL-6Rα splice variant that is nonmembrane-associated. The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway in cells that express gp130 but not IL-6Rα. Research studies have shown that IL-6, through increasing expression of proangiogenic VEGF, may also contribute to metastatic breast cancer. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | E. coli-derived recombinant equine IL‑6. Phe26-Met208. Q95181. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (0.1 µg/mL); Immunofluorescence (5-15 µg/mL); Neutralization (0.5-1.5 µg/mL) |
| MW(KDa) | 21-28 |
| Reactivity | Equine |
| Specificity | Detects equine IL‑6 in direct ELISAs and Western Blots. In direct ELISAs and Western Blots, approximately 50% cross-reactivity with recombinant human IL-6, recombinant porcine IL-6 and recombinant canine IL-6 is observed and approximately 20% cross-reactivity with recombinant mouse IL-6 and recombinant rat IL-6 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.