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IL-33 Antibody
SPA-05886
| Size | Price |
| 0.1 mg | Online Inquiry |
| 0.025 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IL-33 |
| Gene Abbr. | IL33 |
| Gene ID | 90865 |
| Full Name | interleukin 33 |
| Alias | C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV |
| Introduction | Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | A 17 amino acid synthetic peptide from near the center of human IL-33. The immunogen is located within amino acids 150 - 200 of IL-33. Amino Acid Squence: YESQHPSNESGDGVDGK. |
| Usage | |
|---|---|
| Application | WB, ELISA, IF, IHC |
| Dilutions | Western Blot (1-2 µg/mL); ELISA (1:100-1:2000); Immunofluorescence (20 µg/mL); Immunohistochemistry (2.5 µg/mL) |
| Reactivity | Human, Mouse, Rat |
| Specificity | Human IL-33 has 4 isoforms, including isoform 1 (270aa, 30.8kD), isoform 2 (228aa, 25.9kD), isoform 3 (228aa, 26.1kD) and isoform 4 (144aa, 16.3kD). This antibody detects only human isoform L. Mouse IL-33 has 1 isoform (266aa, 30kD). Rat IL-33 has only one isoform identified so far (264aa, 29.5kD). |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.