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IL-18R alpha/IL-1 R5 Antibody
SPA-05710
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IL-18 Receptor |
| Gene Abbr. | IL18R1 |
| Gene ID | 8809 |
| Full Name | interleukin 18 receptor 1 |
| Alias | CD218a, CDw218a, IL-18R-alpha, IL-18Ralpha, IL-1Rrp |
| Introduction | Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5. Glu20-Arg329. Accession # Q13478. |
| Usage | |
|---|---|
| Application | WB, FC, IHC |
| Dilutions | Western Blot (0.1 µg/mL); Flow Cytometry (2.5 µg/10^6 cells); Immunohistochemistry (5-15 µg/mL); Neutralization (0.1-0.4 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human IL‑18 R alpha /IL-1 R5 in direct ELISAs and Western Blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑18 R alpha is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.