IL-1 Sandwich ELISA Kit - CD BioSciences

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IL-1 Sandwich ELISA Kit

IL-1 Sandwich ELISA Kit

SPK-00572

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1 X 96T Online Inquiry
5 X 96T Online Inquiry
10 X 96T Online Inquiry
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Target Information
Target Name IL-1
Gene Abbr. IL1A
Gene ID 100009250
Full Name interleukin 1 alpha
Introduction IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.
Product Details
Measurement Sandwich
Usage
Application ELISA
Reactivity
Assay Principle quantitative
Sensitivity 3.9 pg/mL
Detection Range 15.6 pg/mL - 1000 pg/mL
Sample Volume 50-100μL
Assay Time 1-5h
Detection Wavelength 450 nm
Precision Intra-assay Precision (Precision within an assay): CV%<8%.
Inter-assay Precision (Precision between assays): CV%<10%.
Storage & Handling
Storage Temp. Store at 2-8°C. Please refer to protocol.

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