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IL-1 RII Antibody
SPA-05549
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IL-1 Receptor |
| Gene Abbr. | IL1R2 |
| Gene ID | 7850 |
| Full Name | interleukin 1 receptor type 2 |
| Alias | CD121b, CDw121b, IL-1R-2, IL-1RT-2, IL-1RT2 |
| Introduction | The Interleukin 1 receptor type I (IL-1R1) belongs to the IL-1 receptor family. IL-1R1 binds to IL-1 alpha, IL-1 beta, and interleukin-1 receptor antagonist protein (IL-1RA). IL-1RA regulates IL-1 ligand biological activity by a competitive interaction for IL-1R1. Upon IL-1 stimulation, adaptor molecule MyD88 is first recruited to the IL-1R1-IL1R accessory protein complex, followed by the recruitment of two serine-threonine kinases, IRAK4 and IRAK, and the adaptor protein TRAF6, resulting in activation of the NF-kB pathway. Stimulation of IL-1R1, leads to activation of the transcription factors NF-B, ATF, and AP-1. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 34141 |
| Isotype | IgG1 |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RII. Phe14-Glu343 (Ser56Gly and Glu297Gly). Accession # P27930. |
| Usage | |
|---|---|
| Application | WB, ELISA |
| Dilutions | Western Blot (1 µg/mL); ELISA Capture (2-8 µg/mL); ELISA Detection (0.1-0.4 µg/mL); Neutralization (5-20 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human IL-1 RII in direct ELSAs and Western Blots. When used in combination with the biotinylated human IL-1 RII affinity purified polyclonal detection antibody in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) IL-1ra, rhIL-1 RI, recombinant mouse IL-1ra, or recombinant rat IL-1ra. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.