IL-1 ELISA Kit - CD BioSciences

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IL-1 ELISA Kit

IL-1 ELISA Kit

SPK-00556

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5 X 96T Online Inquiry
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Target Information
Target Name IL-1
Gene Abbr. IL1A
Gene ID 281250
Full Name interleukin 1 alpha
Introduction IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.
Product Details
Measurement Competitive
Usage
Application ELISA
Reactivity
Assay Principle quantitative
Sensitivity 250 pg/mL
Detection Range 250 pg/mL - 4000 pg/mL
Sample Volume 50-100μL
Assay Time 1-5h
Detection Wavelength 450 nm
Precision Intra-assay Precision (Precision within an assay): CV%<15%.
Inter-assay Precision (Precision between assays): CV%<15%.
Storage & Handling
Storage Temp. Store at 2-8°C. Please refer to protocol.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.