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IL-1 beta/IL-1F2 Antibody
SPA-05532
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IL-1 |
| Gene Abbr. | IL1B |
| Gene ID | 3553 |
| Full Name | interleukin 1 beta |
| Alias | IL-1, IL1-BETA, IL1F2, IL1beta |
| Introduction | IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | E. coli-derived recombinant mouse IL-1 beta /IL-1F2. Val118-Ser269. P10749. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1 µg/mL); Neutralization (2-12 µg/mL) |
| Reactivity | Mouse |
| Specificity | Detects mouse IL-1 beta /IL-1F2 in direct ELISAs and Western Blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant cotton rat IL-1 beta and recombinant rhesus monkey IL-1 beta is observed, approximately 15% cross-reactivity with recombinant rat IL-1 beta and recombinant rabbit IL-1 beta is observed, and less than 10% cross-reactivity with recombinant canine IL-1 beta, recombinant equine IL-1 beta, recombinant guinea pig IL-1 beta, recombinant porcine IL-1 beta, recombinant feline IL-1 beta, and recombinant human IL-1 beta is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.