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IL-1 beta/IL-1F2 Antibody
SPA-05518
| Size | Price |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IL-1 |
| Gene Abbr. | IL1B |
| Gene ID | 3553 |
| Full Name | interleukin 1 beta |
| Alias | IL-1, IL1-BETA, IL1F2, IL1beta |
| Introduction | IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-beta, designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | This antibody was prepared by repeated immunizations with recombinant human IL-1 beta/IL-1F2 produced in E.coli. The MW of the recombinant 153 aa IL-1 beta was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta converting enzyme (ICE, capase-1). |
| Usage | |
|---|---|
| Application | WB, ELISA, IF, IHC, IP |
| Dilutions | Western Blot (1:1000); ELISA (1:500-1:2000); Immunofluorescence (1:10-1:500); Immunohistochemistry (1:100-1:200); Immunoprecipitation (1:400-1:800) |
| Reactivity | Human, Mouse, Rat, Porcine, Ba, Canine, Primate |
| Specificity | Detects IL-1 beta and does not cross react with IL-1 alpha. Weakly detects the non-denatured 31 kDa precursor molecule but strongly detects all of the 17 kDa mature molecule. Additionally this IL-1 beta antibody will bind the denatured 31 kDa precursor molecule with greater affinity than the native form, but will often detect past the 35 kDa mark. This IL-1 beta antibody has also been shown to be useful in neutralization of human and primate IL-1 beta activity in bioassays. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.