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IKKβ Sandwich ELISA Kit
SPK-00550
| Size | Price |
| 1 X 96T | Online Inquiry |
| 5 X 96T | Online Inquiry |
| 10 X 96T | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IKKβ |
| Gene Abbr. | Ikbkb |
| Gene ID | 84351 |
| Full Name | inhibitor of nuclear factor kappa B kinase subunit beta |
| Alias | AIM-1, IKK2 |
| Introduction | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation. |
| Product Details | |
|---|---|
| Measurement | Sandwich |
| Usage | |
|---|---|
| Application | ELISA |
| Reactivity | |
| Assay Principle | quantitative |
| Sensitivity | 7.81 pg/mL |
| Detection Range | 31.25 pg/mL - 2000 pg/mL |
| Sample Volume | 50-100μL |
| Assay Time | 1-5h |
| Detection Wavelength | 450 nm |
| Precision | Intra-assay Precision (Precision within an assay): CV%<8%. Inter-assay Precision (Precision between assays): CV%<10%. |
| Storage & Handling | |
|---|---|
| Storage Temp. | Store at 2-8°C. Please refer to protocol. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.