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IGFBP-3 Antibody
SPA-05434
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IGFBP-3 |
| Gene Abbr. | IGFBP3 |
| Gene ID | 3486 |
| Full Name | insulin like growth factor binding protein 3 |
| Alias | BP-53, IBP3 |
| Introduction | The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked and two potential O-linked glycosylation sites. Human IGFBP-3 is expressed in multiple tissues. The highest expression level is found in the non-paranchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty. Human IGFBP-3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGF-I or IGF-II and the acid-labile subunit (ALS). Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619. Spagnoli, A. and R.G. Rosenfeld (1997) Curr. Op. Endocrinology and Diabetes 4:1. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human IGFBP‑3. Gly28-Lys291. Accession # CAA46087. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (0.1 µg/mL); Immunohistochemistry (5-15 µg/mL); Neutralization (0.75-3.0 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human IGFBP‑3 in direct ELISAs and Western Blots. In direct ELISAs, approximately 10% cross reactivity with recombinant mouse IGFBP-3 is observed, and less than 1% cross-reactivity with recombinant human (rh) IGFBP‑1, rhIGFBP‑2, and rhIGFBP‑4 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.