Igf1r cDNA ORF Clone, Mouse, untagged - CD BioSciences

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Igf1r cDNA ORF Clone, Mouse, untagged

Igf1r cDNA ORF Clone, Mouse, untagged

SPD-06638

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Description
Full length Clone DNA of Mouse insulin-like growth factor I receptor
Target Information
Species Mouse
Target Name IGF Receptor
Gene Abbr. Igf1r
Gene ID 16001
Full Name insulin-like growth factor I receptor
Alias A330103N21Rik, CD221, D930020L01, IGF-, IGF-1R
Introduction Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell types in fetal and postnatal tissues, and which is highly similar in sequence and structure to the insulin receptor. IGF-IR is synthesized as a preproprotein which is proteolytically cleaved into alpha and beta subunits. Receptor assembly involves heterodimerization of two alpha and two beta subunits to generate the heterotetrameric transmembrane receptor. The alpha subunits form the extracellular ligand binding domain; ligand binding by IGF-I or IGF-II initiates autophosphorylation of conserved intracellular residues in the beta subunit kinase domain, leading to kinase activation and subsequent activation of downstream signal transduction pathways (e.g., Akt and MAPK). Enhanced mitogenic signaling through the IGF1R is frequently observed in cancer, making the IGF-IR an important research target in translational oncology.
Product Details
Description Full length Clone DNA of Mouse insulin-like growth factor I receptor
NCBI Ref Seq NM_010513.2
RefSeq ORF Size 4110 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence.
Vector pCMV3-untagged
Promoter Enhanced CMV mammalian cell promoter
Restriction Sites HindIII + XbaI (6.1kb + 4.11kb)
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Ampicillin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

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