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IGF-IR/IGF1R Antibody
SPA-05407
| Size | Price |
| 100 µg | Online Inquiry |
| 25 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IGF Receptor |
| Gene Abbr. | IGF1R |
| Gene ID | 3480 |
| Full Name | insulin like growth factor 1 receptor |
| Alias | CD221, IGFIR, IGFR, JTK13 |
| Introduction | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues. Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 33255 |
| Isotype | IgG1 |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R/IGF1R. Glu31-Asn932. Accession # P08069. |
| Usage | |
|---|---|
| Application | WB, FC, IHC, ELISA, IF |
| Dilutions | Western Blot (1 µg/mL); Flow Cytometry (0.25 µg/10^6 cells); Immunohistochemistry (5-25 µg/mL); ELISA Capture (2-8 µg/mL); ELISA Detection (0.1-0.4 µg/mL); Immunofluorescence (3-25 µg/mL) |
| MW(KDa) | 95 |
| Reactivity | Human, Mouse |
| Specificity | Detects human IGF-I R/IGF1R in sandwich ELISAs and Western Blots. Detects mouse IGF-I R/IGF1R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF‑II, rhIL‑3 R alpha, rhIL‑9 R, and rhTGF‑ beta RII. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.