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HLA DRB1 Antibody
SPA-05190
| Size | Price |
| 0.02 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | HLA DRB1 |
| Gene Abbr. | HLA-DRB1 |
| Gene ID | 3123 |
| Full Name | major histocompatibility complex, class II, DR beta 1 |
| Alias | DRB1, HLA-DR1B, HLA-DRB, SS1 |
| Introduction | HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq] |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | DA2 |
| Isotype | IgG1 |
| Immunogen | The membranes of LKT cells (a B lymphoblastoid cell line). |
| Usage | |
|---|---|
| Application | WB, ELISA, FC, IHC |
| Dilutions | Western Blot (1:100-1:2000); ELISA (1:100-1:2000); Flow Cytometry (1:10-1:1000); Immunohistochemistry (1:10-1:500) |
| Reactivity | Human |
| Specificity | DA2 will detect dimers and the beta chain by Western Blot. DA2 will bind all DR and DP antigens except DR7. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS. |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.