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Glut4 Antibody
SPA-04516
| Size | Price |
| 100 µg | Online Inquiry |
| 25 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Glut4 |
| Gene Abbr. | SLC2A4 |
| Gene ID | 6517 |
| Full Name | solute carrier family 2 member 4 |
| Alias | GLUT4 |
| Introduction | A group of related glucose transporters (Glut1-5 and 7) mediate the facilitated diffusion of glucose in nonepithelial mammalian tissues. Within insulin-responsive tissues such as muscle and fat, Glut1 contributes to basal glucose uptake while Glut4 is responsible for insulin-stimulated glucose transport. Glut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation. Translocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 1F8 |
| Isotype | IgG1 |
| Immunogen | Partially purified vesicles containing Glut4. |
| Usage | |
|---|---|
| Application | WB, IHC, IP, IHC, IF |
| Dilutions | Western Blot (1 µg/mL); Immunohistochemistry (5-25 µg/mL); Immunoprecipitation); Immunofluorescence (8-25 µg/mL) |
| MW(KDa) | 50 |
| Reactivity | Rat |
| Specificity | Recognizes an epitope in the cytoplasmic portion of rat Glut4 and has been shown to bind only to the muscle-adipose isoform of the glucose transporter, which is antigenically unique from the other transport proteins (1-3). Recognizes Glut4 in human, monkey, rat, mouse, and rabbit systems. It does not recognize this protein in canine systems. Its ability to bind to glucose transporter from other species has not been tested. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.