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GIT-1 Antibody
SPA-04475
| Size | Price |
| 100 µg | Online Inquiry |
| 25 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | GIT-1 |
| Gene Abbr. | GIT1 |
| Gene ID | 28964 |
| Full Name | GIT ArfGAP 1 |
| Introduction | G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. GIT-1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 924640 |
| Isotype | IgG2B |
| Immunogen | E. coli-derived recombinant human GIT1. Ser485-Asp636. Q9Y2X7. |
| Usage | |
|---|---|
| Application | WB, ELISA, IHC, FC |
| Dilutions | Western Blot (2 µg/mL); Immunohistochemistry (8-25 µg/mL); Flow Cytometry (0.25 µg/10^6 cells) |
| MW(KDa) | 95 |
| Reactivity | Human, Mouse, Rat |
| Specificity | Detects human GIT1 in direct ELISAs and human, mouse, and rat GIT1 in Western Blots. In sandwich immunoassays, this antibody is specific for human GIT1 when paired with the suggested detection antibody. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.