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GADD153/CHOP Antibody
SPA-02205
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CHOP |
| Gene Abbr. | DDIT3 |
| Gene ID | 1649 |
| Full Name | DNA damage inducible transcript 3 |
| Alias | AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10 |
| Introduction | CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner. CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase. Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death. Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum. The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | A synthetic peptide made to an internal portion of the human CHOP/GADD153 protein (between residues 100-150) [UniProt P35638]. |
| Usage | |
|---|---|
| Application | WB, FC, IHC |
| Dilutions | Western Blot (1:1000); Flow Cytometry (2-5 μg/10^6 cells); Immunohistochemistry (1:400) |
| MW(KDa) | 27 |
| Reactivity | Human, Mouse, Rat, Primate |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS (pH 7.4). |
| Preservative | 0.02% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.