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GADD153/CHOP Antibody
SPA-02203
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CHOP |
| Gene Abbr. | DDIT3 |
| Gene ID | 1649 |
| Full Name | DNA damage inducible transcript 3 |
| Alias | AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10 |
| Introduction | CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner. CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase. Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death. Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum. The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | L63F7 |
| Immunogen | Synthetic peptide corresponding to the sequence of human CHOP. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CHOP. |
| Usage | |
|---|---|
| Application | WB, IP, IF, ChIP, FC |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 27 |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of total CHOP protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.