Follistatin-like 4/FSTL4 Antibody - CD BioSciences

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Follistatin-like 4/FSTL4 Antibody

Follistatin-like 4/FSTL4 Antibody

SPA-04069

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Target Information
Target Name Follistatin-like
Gene Abbr. FSTL4
Gene ID 23105
Full Name follistatin like 4
Introduction FSTL4 (Follistatin-like protein 4; also FSL4, SPIG1 and D/Bsp120I-1) is a likely secreted, 90 kDa (predicted) member of the follistatin gene family of TGF-beta superfamily inhibitors. It is widely expressed, being found in neurons (retinal ganglion and cerebellar Purkinje cells), cardiac muscle cells, smooth muscle cells and intestinal epithelium. Mature mouse FSTL4 is 819 amino acids (aa) in length (aa 23-841). It contains one kazal-like domain (aa 80-134), an EF-hand motif (aa 173-208), and two Ig-like domains (aa 250-336 and 340-425). There are two potential splice forms, one that shows an alternative start site at Met315, and a second that possesses a His residue substitution for aa 518-841. Full-length mature mouse FSTL4 shares 95% and 84% aa identity with rat and human FSTL4, respectively.
Product Details
Host Goat
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Chinese hamster ovary cell line CHO-derived recombinant mouse Follistatin-like 4/FSTL4. Trp23-Val841. Accession # Q5STE3.
Usage
Application WB, IHC
Dilutions Western Blot (1 µg/mL); Immunohistochemistry (5-15 µg/mL)
Reactivity Human, Mouse, Rat
Specificity Detects recombinant mouse Follistatin-like 4/FSTL4 in direct ELISAs. Detects human, mouse, and rat Follistatin-like 4/FSTL4 in Western Blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FSTL4 is observed, and less than 5% cross‑reactivity with rhFSTL5 and recombinant mouse FSTL1 is observed.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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