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FLIP Antibody
SPA-04013
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | FLIP |
| Gene Abbr. | CFLAR |
| Gene ID | 8837 |
| Full Name | CASP8 and FADD like apoptosis regulator |
| Alias | CASH, CASP8AP1, CLARP, Casper, FLAME |
| Introduction | Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis that has various names, such as c-FLIP, Casper, CLARP, FLAME, I-FLICE, MRIT, CASH, and Usurpin. FLIP is expressed as two alternative splice isoforms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) like those found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE), and contains an additional death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both FLIP isoforms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some research studies have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease, resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siRNA or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkin's lymphoma and ovarian and colon carcinomas. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP was used as immunogen, GenBank no. gi|12643547. This amino acid sequnce is 100% conserved in FLIP long and short, and various FLIP isoforms as denoted in the NCBI BLAST data base. http://www.ncbi.nlm.nih.gov/blast/. |
| Usage | |
|---|---|
| Application | WB, IHC, IP |
| MW(KDa) | 55, 25 |
| Reactivity | Human |
| Specificity | The gene encoding FLIP produces, by alternative splicing, 24 different transcripts, all with introns, putatively encoding 22 different protein isoforms. The molecular weights of these isoforms may vary. Therefore, the molecular weight of FLIP observed by Western Blot may vary according to the isoforms present in the sample. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Whole antisera. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.