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Fibronectin Antibody
SPA-03980
| Size | Price |
| 0.025 mL | Online Inquiry |
| 0.1 mL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Fibronectin |
| Gene Abbr. | FN1 |
| Gene ID | 2335 |
| Full Name | fibronectin 1 |
| Alias | CIG, ED-B, FINC, FN, FNZ |
| Introduction | Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | DH1 |
| Isotype | IgG1 |
| Immunogen | The antibody reacts with Fibronectin. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (1:100-1:2000); Immunohistochemistry (1:10-1:500) |
| Reactivity | Human, Rat, Chicken, Guinea Pig, Rabbit |
| Specificity | The antibody is specific to extradomain A (EDA) sequence of a cellular fibronectin and recognizes thus only the cellular fibronectin. Mab DHI has been shown to specifically block chondrocyte condensation in chicken embryos. The antibody cross reacts with human, chicken, rat, rabbit and guinea pig. Fibronectins are diverse high molecular weight cell adhesion glycoproteins found in basement membranes, in the interstitial connective tissue matrix, and in soluble in plasma and other body fluids. In adult tissues most fibronectin is produced by hepatocytes (pFn) that differ from locally produced cellular fibronectins. A monoclonal antibody (BF12) has been produced to fibronectin that reacts with both the cellular and plasma forms of the protein and recognizes the cell-binding region in fibronectin. The monoclonal antibody DH1 recognizes only cellular form of Fibronectin. DH1 is specific to the extra domain A (EDA) of the molecule. cFns are found in some basement membranes during development and only in distinct tissues in adult, for instance in smooth muscle and alveolar walls of airways. cFns are abundant in the plasma of carcinoma patients and in the stroma of various carcinomas. Monoclonal antibody (Mab) to cFn is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Purified fibronectin from A8387 fibrosarcoma cells was used as an immunogen. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS containing 1% BSA. |
| Preservative | 0.1% Sodium Azide |
| Storage Temp. | Store at 4 °C. |
| Handling | Do not freeze. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.