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FGF-8 (b Isoform) Antibody
SPA-03886
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | FGF |
| Gene Abbr. | FGF8 |
| Gene ID | 2253 |
| Full Name | fibroblast growth factor 8 |
| Alias | AIGF, FGF-8, HBGF-8, HH6, KAL6 |
| Introduction | FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1‑3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a‑h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5‑8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9‑12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13). Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928. Gemel, J. et al. (1996) Genomics 35:253. Olsen, S.K. et al. (2006) Genes Dev. 20:185. Crossley, P.H. et al. (1996) Cell, 84:127. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129. Sun, X. et al. (1999) Genes Dev. 13:1834. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425. Mattila, M.M. et al. (2001) Oncogene 20:2791. Valve, E. et al. (2000) Int. J. Cancer 88:718. Valve, E.M. et al. (2001) Lab. Invest. 81:815. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.Show all background references |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | E. coli-derived recombinant mouse FGF-8b. Gln23-Arg215. NP_006110. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (0.1 µg/mL); Immunohistochemistry (5-15 µg/mL); Neutralization (0.4-2.4 µg/mL) |
| Reactivity | Mouse |
| Specificity | Detects mouse FGF‑8b Isoform in direct ELISAs and Western Blots. In direct ELISAs and Western Blots, less than 1% cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7 and rhFGF-9 is observed. It does, however, cross‑react with recombinant mouse FGF-8c in neutralizing bioassay. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.