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Fas Antibody
SPA-03807
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Fas |
| Gene Abbr. | FAS |
| Gene ID | 355 |
| Full Name | Fas cell surface death receptor |
| Alias | ALPS1A, APO-1, APT1, CD95, FAS1 |
| Introduction | Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers. Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes. FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8. Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites. FasL is also expressed in several tumor types as a mechanism to evade immune surveillance. Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | C18C12 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Lys259 of human Fas, within the intracellular region. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys259 of human Fas, within the intracellular region. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 40-50 |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of total human Fas protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.