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F4/80 Antibody
SPA-03739
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | F4/80 |
| Gene Abbr. | ADGRE1 |
| Gene ID | 2015 |
| Full Name | adhesion G protein-coupled receptor E1 |
| Alias | EMR1, TM7LN3 |
| Introduction | F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGFTM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells. |
| Product Details | |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Clone No. | 521204 |
| Isotype | IgG2A |
| Immunogen | NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1. Gln28-Gly931. Accession # Q61549. |
| Usage | |
|---|---|
| Application | FC, IHC, IF |
| Dilutions | Flow Cytometry (0.25 µg/10^6 cells); Immunohistochemistry (5-25 µg/mL); Immunofluorescence (8-25 µg/mL) |
| Reactivity | Mouse |
| Specificity | Detects mouse F4/80/EMR1. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.