Online Inquiry
Ephrin-A4 Antibody
SPA-03626
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Ephrin |
| Gene Abbr. | EFNA4 |
| Gene ID | 1945 |
| Full Name | ephrin A4 |
| Alias | EFL4, EPLG4, LERK4 |
| Introduction | Ephrin-A4, also known as LERK-4 and EFL-4, is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation. In vivo, the ligands and receptors display reciprocal expression. It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue. The Eph/ephrin families also appear to play a role in angiogenesis. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Ephrin-A4. Leu26-Gly176. Accession # O08542. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (0.1 µg/mL) |
| Reactivity | Mouse |
| Specificity | Detects mouse Ephrin‑A4 in direct ELISAs and Western Blots. In Western Blots, approximately 35% cross‑reactivity with recombinant human (rh) Ephrin‑A4 is observed and less than 5% cross‑reactivity with rhEphrin-B3, recombinant mouse (rm) Ephrin‑A1, rmEphrin-A2, rhEphrin-A5, and rhEphrin-A3 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.