EphA5 Antibody - CD BioSciences

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EphA5 Antibody

EphA5 Antibody

SPA-03518

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Target Information
Target Name EphA5
Gene Abbr. EPHA5
Gene ID 2044
Full Name EPH receptor A5
Alias CEK7, EHK-1, EHK1, EK7, HEK7
Introduction This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq]
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 86731
Isotype IgG1
Immunogen Mouse myeloma cell line NS0-derived recombinant rat EphA5. Ser58-Gln573 (Asp170Glu). Accession # P54757.
Usage
Application WB, FC, IF
Dilutions Western Blot (1 µg/mL); Flow Cytometry (0.25 µg/10^6 cells); Immunofluorescence (8-25 µg/mL)
Reactivity Human, Rat
Specificity Detects rat EphA5 in direct ELISAs and Western Blots. In direct ELISAs, 5‑15% cross reactivity with recombinant mouse (rm) EphA4 and rmEpha8 is observed and no cross-reactivity with recombinant human (rh) EphA1, rmEphA2, -A3, -A6, -A7, recombinant rat EphB1, -B2, or rmEphB3 is observed.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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