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eEF1A1 Antibody
SPA-03222
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | eEF1A |
| Gene Abbr. | EEF1A1 |
| Gene ID | 1915 |
| Full Name | eukaryotic translation elongation factor 1 alpha 1 |
| Alias | CCS-3, CCS3, EE1A1, EEF-1, EEF1A |
| Introduction | Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells. eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome. After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B. Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to a sequence at the C-terminal end of human eEF1A. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence at the C-terminal end of human eEF1A. Antibodies are purified by peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 50 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of total eEF1A protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.