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EBI3 Antibody
SPA-03194
| Size | Price |
| 0.025 mg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | EBI3 |
| Gene Abbr. | EBI3 |
| Gene ID | 10148 |
| Full Name | Epstein-Barr virus induced 3 |
| Alias | IL-27B, IL27B, IL35B |
| Introduction | EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively (Honglian Tong et al, 2010). IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis (Kerstin A et al, 2008). |
| Product Details | |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Clone No. | 355022 |
| Isotype | IgG2A |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer. Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28). Accession # O35228 (EBI3) & Q8K3I6 (p28). |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (2 µg/mL) |
| Reactivity | Mouse |
| Specificity | Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western Blots. In direct ELISAs and Western Blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.