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E2F-4 Antibody
SPA-03182
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | E2F-4 |
| Gene Abbr. | E2F4 |
| Gene ID | 1874 |
| Full Name | E2F transcription factor 4 |
| Alias | E2F-4 |
| Introduction | The E2F family consists of 8 transcription factors that regulate genes that control cell cycle progression by complexing with DP and Rb proteins. E2F transcriptional activation is generally opposed by associating with RB proteins, pRB, p107, and p130. E2F-1, -2, and -3a function as activators that can help quiescent cells enter S phase, while E2F-3b, -4, and -5 repress cell growth through the recruitment of HDAC’s and other corepressors to target genes. E2F-6 diverges considerably from other family members, and has repressive properties governed not through interaction with Rb proteins, but by recruiting the polycomb repressive complex. E2F-7, and -8 are unique in that they have two DNA-binding domains and do not heterodimerize with DP proteins. These E2F family members repress transcription and delay progression of the cell cycle through the regulation of E2F-1 (13-15) |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | E3G2G |
| Immunogen | Synthetic peptide corresponding to residues surrounding Leu405 of human E2F4 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu405 of human E2F4 protein. |
| Usage | |
|---|---|
| Application | WB, IP, ChIP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 62 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total E2F4 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.