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E2A Antibody
SPA-03180
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | E2A |
| Gene Abbr. | TCF3 |
| Gene ID | 6929 |
| Full Name | transcription factor 3 |
| Alias | AGM8, E2A, E47, ITF1, TCF-3 |
| Introduction | E2A is a member of the E-protein family of transcription factors, a subclass of basic helix-loop-helix (bHLH) proteins that bind specifically to E-box consensus sequences. Alternative splicing generates two E2A isoforms (E47 and E12) that are actively involved in B cell lineage commitment, B cell maturation, IgK V-J rearrangement, peripheral B cell development, and tumor suppression. E2A acts in cis during G1 to promote immunoglobulin gene diversification. Research studies have shown that chromosomal translocations involving the E2A gene result in the expression of multiple fusion proteins and are associated with many cases of pediatric acute lymphoblastic leukemia. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D2B1 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Leu475 of human E2A protein. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu475 of human E2A protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 75 |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | It detects endogenous levels of total E2A protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.